Proteintech, 21506-1-AP, C1orf106 Polyclonal antibody

Size: 20ul / 150ul The C1orf106 (21506-1-AP) by Proteintech is a Polyclonal antibody targeting C1orf106 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 21506-1-AP targets C1orf106 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse kidney tissue, HEK-293 cells, rat kidney tissue Positive IP detected in: mouse kidney tissue Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information C1orf106 (also known as innate immunity activator, INAVA) has thus far been considered to play a significant role in intestinal inflammatory diseases. C1orf106 is highly expressed in the human intestine and intestinal epithelial cell lines but expressed at low levels in myeloid cells and mouse bone marrow-derived macrophages (PMID: 29420262). Recent studies have also shown that C1orf106 protects against colitis by stabilizing E-cadherin and bridges mucosal barrier function with inflammatory signaling (PMID: 31376015). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15987 Product name: Recombinant human C1orf106 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 314-663 aa of BC106877 Sequence: PERAPVQNSPWKETSLDHPYEKPRKSSEPWSESSSPATTPQDGPSASSLWLLEPASYHVVPIRGVPGQWQGRTSAPATPEIQGRRGQSQSLRVDSFRAGPEGRGRSAFPRRRPTHYTVTVPDSCFPATKPPLPHAACHSCSEDSGSDVSSISHPTSPGSSSPDISFLQPLSPPKTHRHRGAWVPAGSRELVAHHPKLLLPPGYFPAGRYVVVAESPLPPGEWELCRAAPGPAYEEEGTPLRYQRLVPSRSRIVRTPSLKDSPAGRGLSKAAVSEELKWWHERARLRSTRPHSLDRQGAFRVRSLPLGREGFGRALGPRAQVPTVCVLRRSPDGAPVQVFVPEKGEIISQV Predict reactive species Full Name: chromosome 1 open reading frame 106 Calculated Molecular Weight: 663 aa, 73 kDa Observed Molecular Weight: 70-73 kDa GenBank Accession Number: BC106877 Gene Symbol: C1orf106 Gene ID (NCBI): 55765 RRID: AB_10734431 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q3KP66 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.