Proteintech, 21524-1-AP, CCT4 Polyclonal antibody

Size: 20ul / 150ul The CCT4 (21524-1-AP) by Proteintech is a Polyclonal antibody targeting CCT4 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 21524-1-AP targets CCT4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, HEK-293 cells, HeLa cells, mouse heart tissue Positive IHC detected in: human liver cancer tissue, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information CCT4 (Chaperonin containing TCP1 complex 4), also known as TCP-1-delta, is a recent identified member of the family, that serves as a component of the chaperonin-containing TCP1 complex (TRiC) and also functions as a monomer. CCT4 has been shown to influence the cytoskeletal structure and regulate F-actin organization independently of TRiC (PMID: 41104595). CCT4 was highly expressed in human ESCC tissues and cell lines, and closely related to the poor prognosis (PMID: 36102200). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16128 Product name: Recombinant human CCT4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 108-407 aa of BC106934 Sequence: SVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHD Predict reactive species Full Name: chaperonin containing TCP1, subunit 4 (delta) Calculated Molecular Weight: 539 aa, 58 kDa Observed Molecular Weight: 58-60 kDa GenBank Accession Number: BC106934 Gene Symbol: CCT4 Gene ID (NCBI): 10575 RRID: AB_10733520 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P50991 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.