Proteintech, 21677-1-AP, PLEKHG6 Polyclonal antibody

Size: 20ul / 150ul The PLEKHG6 (21677-1-AP) by Proteintech is a Polyclonal antibody targeting PLEKHG6 in WB, ELISA applications with reactivity to human samples 21677-1-AP targets PLEKHG6 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Caco-2 cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information PLEKHG6 is a previously unknown guanine nucleotide exchange factor (GEF) for RhoG. It localizes to the apical membrane of cells, a process that depends on its interaction with the membrane-cytoskeleton linker protein ezrin. This apical localization is essential for inducing morphological changes. Additionally, PLEKHG6 and ezrin are both necessary for macropinocytosis mediated by membrane ruffles. Finally, ezrin facilitates precise spatial regulation of RhoG activation at the apical surface of epithelial cells by recruiting components of the RhoG signaling pathway into a closely associated complex. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15886 Product name: Recombinant human PLEKHG6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 456-790 aa of BC105961 Sequence: LMLEKLVCQPLRDPNSFLLIHLTEFQCVSSALLVHCPSPTDRAQWLEKTQQAQAALQKLKAEEYVQQKRELLTLYRDQDRESPSTRPSTPSLEGSQSSAEGRTPEFSTIIPHLVVTEDTDEDAPLVPDDTSDSGYGTLIPGTPTGSRSPLSRLRQRALRRDPRLTFSTLELRDIPLRPHPPDPQAPQRRSAPELPEGILKGGSLPQEDPPTWSEEEDGASERGNVVVETLHRARLRGQLPSSPTHADSAGESPWESSGEEEEEGPLFLKAGHTSLRPMRAEDMLREIREELASQRIEGAEEPRDSRPRKLTRAQLQRMRGPHIIQLDTPLSASEV Predict reactive species Full Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 6 Calculated Molecular Weight: 790 aa, 89 kDa Observed Molecular Weight: 89 kDa GenBank Accession Number: BC105961 Gene Symbol: PLEKHG6 Gene ID (NCBI): 55200 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q3KR16 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.