Product Description
Size: 20ul / 150ul
The Trehalase (21685-1-AP) by Proteintech is a Polyclonal antibody targeting Trehalase in WB, IF-P, ELISA applications with reactivity to human, mouse samples
21685-1-AP targets Trehalase in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: mouse small intestine tissue
Positive IF-P detected in: human kidney tissue
Recommended dilution
Western Blot (WB): WB : 1:500-1:3000
Immunofluorescence (IF)-P: IF-P : 1:50-1:500
Background Information
Trehalase is the enzyme hydrolyzing trehalose and belongs to the glycosyl hydrolase 37 family. In humans and most mammals, trehalase is found in the brush borders of the intestinal mucosa, kidney, liver, and blood plasma. Trehalases are conserved enzymes that catalyze the hydrolysis of one of two glycoside bonds of trehalose. With the exception of vertebrates where trehalase is required for the degradation of ingested trehalose, in other animal groups it is involved in diverse physiological processes including carbohydrate metabolism, resumption of growth in certain resting cells, germination of spores in fungi, recovery from stress conditions, etc (PMID: 25429048, PMID: 30628830).
Specification
Tested Reactivity: human, mouse
Cited Reactivity: mouse
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Immunogen: CatNo: Ag16246 Product name: Recombinant human TREH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 203-552 aa of BC109206 Sequence: YLTHTNDTAFLQENIETLALELDFWTKNRTVSVSLEGKNYLLNRYYVPYGGPRPESYSKDVELADTLPEGDREALWAELKAGAESGWDFSSRWLIGGPNPNSLSGIRTSKLVPVDLNAFLCQAEELMSNFYSRLGNDSQATKYRILRSQRLAALNTVLWDEQTGAWFDYDLEKKKKNREFYPSNLTPLWAGCFSDPGVADKALKYLEDNRILTYQYGIPTSLQKTGQQWDFPNAWAPLQDLVIRGLAKAPLRRAQEVAFQLAQNWIRTNFDVYSQKSAMYEKYDVSNGGQPGGGGEYEVQEGFGWTNGVVLMLLDRYGDRLTSGAKLAFLEPHCLAATLLPSLLLSLLPW Predict reactive species
Full Name: trehalase (brush-border membrane glycoprotein)
Calculated Molecular Weight: 583 aa, 67 kDa
Observed Molecular Weight: 67-70 kDa
GenBank Accession Number: BC109206
Gene Symbol: Trehalase
Gene ID (NCBI): 11181
Conjugate: Unconjugated
Form: Liquid
Purification Method: Antigen Affinity purified
UNIPROT ID: O43280
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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