Proteintech, 21717-1-AP, ZIC3 Polyclonal antibody

Size: 20ul / 150ul The ZIC3 (21717-1-AP) by Proteintech is a Polyclonal antibody targeting ZIC3 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 21717-1-AP targets ZIC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Neuro-2a cells, mouse cerebellum tissue, SH-SY5Y cells, Y79 cells Positive IHC detected in: human urothelial carcinoma tissue, human thyroid cancer tissue, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16354 Product name: Recombinant human ZIC3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC113395 Sequence: MTMLLDGGPQFPGLGVGSFGAPRHHEMPNREPAGMGLNPFGDSTHAAAAAAAAAAFKLSPAAAHDLSSGQSSAFTPQGSGYANALGHHHHHHHHHHHTSQVPSYGGAASAAFNSTREFLFRQRSSGLSEAASGGGQHGLFAGSASSLHAPAGIPEPPSYLLFPGLHEQGAGHPSPTGHVDNNQVHLGLRGELFGRADPYR Predict reactive species Full Name: Zic family member 3 (odd-paired homolog, Drosophila) Calculated Molecular Weight: 467 aa, 51 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC113395 Gene Symbol: ZIC3 Gene ID (NCBI): 7547 RRID: AB_2878910 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60481 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.