Proteintech, 21997-1-AP, CD107a / LAMP1 Polyclonal antibody

Size: 20ul / 150ul The CD107a / LAMP1 (21997-1-AP) by Proteintech is a Polyclonal antibody targeting CD107a / LAMP1 in WB, IHC, IF-P, ELISA applications with reactivity to human samples 21997-1-AP targets CD107a / LAMP1 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, HEK-293 cells, L02 cells, MCF-7 cells, MDA-MB-231 cells, U-937 cells Positive IHC detected in: human colon cancer tissue, human gliomas tissue, human liver cancer tissue, human pancreas tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human ovary tumor tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis. Specification Tested Reactivity: human Cited Reactivity: human, pig, monkey, zebrafish, bovine, turtle Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17367 Product name: Recombinant human CD107a protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 35-377 aa of BC021288 Sequence: NGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLL Predict reactive species Full Name: lysosomal-associated membrane protein 1 Calculated Molecular Weight: 45 kDa Observed Molecular Weight: 90-120 kDa GenBank Accession Number: BC021288 Gene Symbol: LAMP1 Gene ID (NCBI): 3916 ENSEMBL Gene ID: ENSG00000185896 RRID: AB_2878966 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P11279 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.