Proteintech, 22028-1-AP, PIK3C2A Polyclonal antibody

Size: 20ul / 150ul The PIK3C2A (22028-1-AP) by Proteintech is a Polyclonal antibody targeting PIK3C2A in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 22028-1-AP targets PIK3C2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, PC-3 cells, human placenta tissue Positive IP detected in: PC-3 cells Positive IHC detected in: mouse ovary tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Specification Tested Reactivity: human, mouse Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16894 Product name: Recombinant human PIK3C2A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-338 aa of BC113658 Sequence: MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRGQWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQGSLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPLSKPKVDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVTRSQS Predict reactive species Full Name: phosphoinositide-3-kinase, class 2, alpha polypeptide Calculated Molecular Weight: 1686 aa, 191 kDa Observed Molecular Weight: 191 kDa GenBank Accession Number: BC113658 Gene Symbol: PIK3C2A Gene ID (NCBI): 5286 RRID: AB_11183760 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O00443 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.