Proteintech, 22050-1-AP, CEP97 Polyclonal antibody

Size: 20ul / 150ul The CEP97 (22050-1-AP) by Proteintech is a Polyclonal antibody targeting CEP97 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, canine samples 22050-1-AP targets CEP97 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, canine samples. Tested Applications Positive WB detected in: HL-60 cells, HEK-293 cells, HeLa cells, PC-3 cells Positive IP detected in: HeLa cells Positive IHC detected in: human breast cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MDCK cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97. Specification Tested Reactivity: human, canine Cited Reactivity: human, mouse, drosophila Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16995 Product name: Recombinant human CEP97 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 506-865 aa of BC041085 Sequence: ESTEQKQSDIKKPENTQPENKETISQATSEKLPMILTQRSVALGQDKVALQKLNDAATKLQACWRGFYARNYNPQAKDVRYEIRLRRMQEHIVCLTDEIRRLRKERDEERIKKFVQEEAFRFLWNQVRSLQVWQQTVDQRLSSWHTDVPPISSTLVPSKHPLFTQSQESSCDQNADWFIASDVAPQEKSLPEFPDSGFHSSLTEQVHSLQHSLDFEKSSTEGSESSIMGNSIDTVRYGKESDLGDVSEEHGEWNKESSNNEQDNSLLEQYLTSVQQLEDADERTNFDTETRDSKLHIACFPVQLDTLSDGASVDESHGISPPLQGEISQTQENSKLNAEVQGQQPECDSTFQLLHVGVTV Predict reactive species Full Name: centrosomal protein 97kDa Calculated Molecular Weight: 865 aa, 97 kDa Observed Molecular Weight: 130-140 kDa GenBank Accession Number: BC041085 Gene Symbol: CEP97 Gene ID (NCBI): 79598 RRID: AB_11182378 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IW35 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.