Proteintech, 22094-1-AP, TLE3 Polyclonal antibody

Size: 20ul / 150ul The TLE3 (22094-1-AP) by Proteintech is a Polyclonal antibody targeting TLE3 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 22094-1-AP targets TLE3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, A431 cells, HepG2 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human lung cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200 Background Information Transducin-like enhancer of split 3(TLE3) is a corepressor that binds to a variant of transciption factor, which mediated by CTNNB1 and TCF family members in Wnt signaling. It's suggested that effect of full-length TLE family members be regulated by association with dominant-negative AES. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17300 Product name: Recombinant human TLE3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 158-240 aa of BC015729 Sequence: PVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHRGSADYSMEAKKRKAEEKDSLSRYDS Predict reactive species Full Name: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) Calculated Molecular Weight: 772 aa, 83 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: BC015729 Gene Symbol: TLE3 Gene ID (NCBI): 7090 RRID: AB_2878993 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q04726 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.