Proteintech, 22147-1-AP, ADAM5 Polyclonal antibody

Size: 20ul / 150ul The ADAM5 (22147-1-AP) by Proteintech is a Polyclonal antibody targeting ADAM5 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 22147-1-AP targets ADAM5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ADAM5(putative disintegrin and metalloproteinase domain-containing protein 5) is also named as ADAM5P, TMDC2(tMDCII). The tMDC II, abundantly expressed, sperm surface MDC proteins identified in rodent and macaque systems, is also nonfunctional in the human(PMID:10417343). It has 7 isoforms produced by alternative splicing. It is expressed in testis highly by northern blot. This antibody is specific to ADAM5. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17590 Product name: Recombinant human ADAM5P protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 183-412 aa of BC067864 Sequence: YCNGDLPYCLPDTYVRDGEYCDSGGAFCFQGKCRTFDKQCDDLIGRGSRGAPVFCYDEINTRGDNFGNCGTAHCLFQHILCGKLVCTWEHRDLISRPNLSVIYAHVRDQTCVSTYLPRRTPPPVNSPISITSYYSAEDRDETFVQDGSMCGPDMYCFEMHCKHVRFLMNLKLCDASNHCDRHGVCNNFNHCHCEKGYNPPYCQPKQGAFGSIDDGHLVPPTERSYMEEGR Predict reactive species Full Name: ADAM metallopeptidase domain 5 pseudogene Calculated Molecular Weight: 412 aa, 47 kDa Observed Molecular Weight: 36-50 kDa GenBank Accession Number: BC067864 Gene Symbol: ADAM5/ADAM5P Gene ID (NCBI): 255926 RRID: AB_11182171 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.