Proteintech, 22261-1-AP, ESPL1 Polyclonal antibody

Size: 20ul / 150ul The ESPL1 (22261-1-AP) by Proteintech is a Polyclonal antibody targeting ESPL1 in WB, ELISA applications with reactivity to human samples 22261-1-AP targets ESPL1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, Jurkat cells, Raji cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information ESPL1 (Extra spindle poles-like 1 protein), also known as Separin, Separase, a cysteine endopeptidase, plays a vital role in the stable binding between sister chromatids before anaphase and their timely separation during anaphase, which is the key to chromosome inheritance (PMID: 34512713). ESPL1 expression is elevated and predicts poor outcomes in patients with various types of cancer, including breast cancer, glioma and endometrial cancer (PMID: 36465268). Full-length ESPL1 decreased after 30 min resulting in a ~ 170 kDa N-terminal (ESPL1N−term) and a ~ 60 kDa C-terminal polypeptide (ESPL1CC−term), reflecting ESPL1 activation and auto-cleavage (PMID: 30429481) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: extra spindle pole bodies homolog 1 (S. cerevisiae) Calculated Molecular Weight: 233 kDa Observed Molecular Weight: 170 kDa GenBank Accession Number: NM_012291 Gene Symbol: ESPL1 Gene ID (NCBI): 9700 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14674 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.