Proteintech, 22291-1-AP, SAR1A Polyclonal antibody

Size: 20ul / 150ul The SAR1A (22291-1-AP) by Proteintech is a Polyclonal antibody targeting SAR1A in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 22291-1-AP targets SAR1A in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, HT-1080 cells, HepG2 cells, mouse kidney tissue, HEK-293T cells, HeLa cells Positive IHC detected in: mouse skeletal muscle tissue, human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SAR1A, also named as SAR1, SARA and SARA1, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1A is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). The antibody is specific to SAR1A. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: SAR1 homolog A (S. cerevisiae) Calculated Molecular Weight: 22 kDa Observed Molecular Weight: 23-25 kDa GenBank Accession Number: NM_020150 Gene Symbol: SAR1A Gene ID (NCBI): 56681 RRID: AB_2879062 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NR31 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.