Proteintech, 22340-1-AP, USP11 Polyclonal antibody

Size: 20ul / 150ul The USP11 (22340-1-AP) by Proteintech is a Polyclonal antibody targeting USP11 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 22340-1-AP targets USP11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, A549 cells, HEK-293 cells, human testis tissue Positive IP detected in: HEK-293 cells Positive IHC detected in: human breast cancer tissue, human brain tissue, human liver cancer tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information USP11(Ubiquitin carboxyl-terminal hydrolase 11) is also named as UHX1 and belongs to the peptidase C19 family. It is a deubiquitinating enzyme that controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17829 Product name: Recombinant human USP11 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 441-743 aa of BC000350 Sequence: YLSVPLLISHKRVLEVFFIPMDPRRKPEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLSSILDRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLMLFGHPLLVSVPRDRFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDDEEDKDDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRRRRKQLFTLQTVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGYVKHDCVGYVMKKAPVRLQECI Predict reactive species Full Name: ubiquitin specific peptidase 11 Calculated Molecular Weight: 963 aa, 110 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: BC000350 Gene Symbol: USP11 Gene ID (NCBI): 8237 RRID: AB_2879079 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P51784 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.