Proteintech, 22661-1-AP, SLC30A1 Polyclonal antibody

Size: 20ul / 150ul The SLC30A1 (22661-1-AP) by Proteintech is a Polyclonal antibody targeting SLC30A1 in WB, ELISA applications with reactivity to human samples 22661-1-AP targets SLC30A1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HeLa membrane protein, HepG2 total crude protein, HepG2 membrane protein Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Zinc transporter 1 (ZNT1), also known as SLC30A1, is the only zinc transporter predominantly located on the plasma membrane, where it plays a pivotal role exporting cytosolic zinc to the extracellular space. SLC30A1 expression is posttranslationally regulated by cellular zinc status and can be N-glycosylated on Asn299 in the extracellular loop between transmembrane domains (PMID: 15451416, 18936201). ZnT1 is located in the plasma membrane and is responsible for Zn export from the cytosol to the extracellular space during periods of elevated cytosolic Zn. Western analysis detected a higher band at∼63 kDa than the expected molecular mass, which could be attributed to the properties of the cytosolic histidine-rich loop (PMID: 31471319, 15753530). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18567 Product name: Recombinant human SLC30A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 330-507 aa of BC121015 Sequence: YTTYPLLKESALILLQTVPKQIDIRNLIKELRNVEGVEEVHELHVWQLAGSRIIATAHIKCEDPTSYMEVAKTIKDVFHNHGIHATTIQPEFASVGSKSSVVPCELACRTQCALKQCCGTLPQAPSGKDAEKTPAVSISCLELSNNLEKKPRRTKAENIPAVVIEIKNMPNKQPESSL Predict reactive species Full Name: solute carrier family 30 (zinc transporter), member 1 Calculated Molecular Weight: 507 aa, 55 kDa Observed Molecular Weight: 63~75 kDa GenBank Accession Number: BC121015 Gene Symbol: SLC30A1 Gene ID (NCBI): 7779 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y6M5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.