Proteintech, 22786-1-AP, SAMD9 Polyclonal antibody

Size: 20ul / 150ul The SAMD9 (22786-1-AP) by Proteintech is a Polyclonal antibody targeting SAMD9 in WB, IHC, ELISA applications with reactivity to human samples 22786-1-AP targets SAMD9 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Sterile alpha motif domain-containing protein 9(SAMD9), aslo named as C7orf5, DRIF1, KIAA2004, OEF1, plays a role in growth factor signal transduction. SAMD9 is a ubiquitously expressed cytoplasmic protein encoded by two juxtaposed single-exon genes on chromosome 7q21(PMID: 34675305). Overexpression of SAMD9 protein in tissue culture results in reduction of cell proliferation rate and invasion index, and increasing activity of caspase 3(PMID: 25450373). An important paralog of this gene is SAMD9L. Observed MW of SAMD9 is from 148 kDa(PMID: 21248034). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18787 Product name: Recombinant human SAMD9 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-178 aa of BC132775 Sequence: MAKQLNLPENTDDWTKEDVNQWLESHKIDQKHREILTEQDVNGAVLKWLKKEHLVDMGITHGPAIQIEELFKELRKTAIEDSIQTSKMGKPSKNAPKDQTVSQKERRETSKQKQKGKENPDMANPSAMSTTAKGSKSLKVELIEDKIDYTKERQPSIDLTCVSYPFDEFSNPYRYKLD Predict reactive species Full Name: sterile alpha motif domain containing 9 Calculated Molecular Weight: 1589 aa, 184 kDa Observed Molecular Weight: 148 kDa GenBank Accession Number: BC132775 Gene Symbol: SAMD9 Gene ID (NCBI): 54809 RRID: AB_2879168 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5K651 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.