Proteintech, 22819-1-AP, PRR14 Polyclonal antibody

Size: 20ul / 150ul The PRR14 (22819-1-AP) by Proteintech is a Polyclonal antibody targeting PRR14 in WB, IHC, ELISA applications with reactivity to human, mouse samples 22819-1-AP targets PRR14 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HCT 116 cells, HL-60 cells, MCF-7 cells, SW480 cells, mouse brain tissue, mouse skeletal muscle tissue Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information PRR14 (proline-rich protein 14) is a 585-amino acid protein with a calculated molecular weight of 64 kDa. This protein functions to tether heterochromatin to the nuclear periphery during interphase, through associations with heterochromatin protein 1 (HP1) and the nuclear lamina. After disassembly early in mitosis, PRR14 reassembles in two steps, first binding to anaphase chromosomes through HP1, followed by association with the nuclear lamina in telophase. PRR14 may thereby play a role in specifying HP1-bound heterochromatin for reattachment to the nuclear lamina at mitotic exit. (PMID: 24209742; 24637393) Specification Tested Reactivity: human, mouse Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14119 Product name: Recombinant human PRR14 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 240-585 aa of BC000119 Sequence: RLSPWGLAPLFRSVRSKLESFADIFLTPNKTPQPPPPSPPMKLELKIAISEAEQSGAAEGTASVSPRPPIRQWRTQDHNTPALLPKPSLGRSYSCPDLGPPGPGTCTWPPAPPQPSRPRPRRHTVGGGEMARAPPPPRPCLRKEVFPLGGVGASPSLTTSCSSTASTSFSEPAEPRLGSTKGKEPRASKDQVLSEPETKTMGKVSRFRIRRTPARPQLNLTPMGLPRPIRLNKKEFSLEEIYTNKNYQSPTTRRTFETIFEEPRERNGTLIFTSSRKLRRAVEFRDSSLPRSRRPSRGVRAAGGRTVPPNVAPSPDVGPLLQQRLEELDALLLEEETVDREQPHWT Predict reactive species Full Name: proline rich 14 Calculated Molecular Weight: 585 aa, 64 kDa Observed Molecular Weight: 64-70 kDa GenBank Accession Number: BC000119 Gene Symbol: PRR14 Gene ID (NCBI): 78994 RRID: AB_2879174 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BWN1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.