Proteintech, 23138-1-AP, GLIS1 Polyclonal antibody

Size: 20ul / 150ul The GLIS1 (23138-1-AP) by Proteintech is a Polyclonal antibody targeting GLIS1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 23138-1-AP targets GLIS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells Positive IHC detected in: mouse ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information GLIS1 is a nuclear zinc finger transcription factor that integrates developmental signaling and transcriptional control during neural and epithelial differentiation. It enhances cellular reprogramming efficiency and modulates pathways linked to Hedgehog and Wnt signaling. Dysregulated GLIS1 expression contributes to cardiac malformations, cancer progression, and stemness reactivation, highlighting its dual role in developmental regulation and disease pathogenesis. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19214 Product name: Recombinant human GLIS1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 360-620 aa of BC101799 Sequence: GPDTEADVLTECLVLQQLHTSTQLAASDGKGGCGLGQELLPGVYPGSITPHNGLASGLLPPAHDVPSRHHPLDATTSSHHHLSPLPMAESTRDGLGPGLLSPIVSPLKGLGPPPLPPSSQSHSPGGQPFPTLPSKPSYPPFQSPPPPPLPSPQGYQGSFHSIQSCFPYGDCYRMAEPAAGGDGLVGETHGFNPLRPNGYHSLSTPLPATGYEALAEASCPTALPQQPSEDVVSSGPEDCGFFPNGAFDHCLGHIPSIYTDT Predict reactive species Full Name: GLIS family zinc finger 1 Calculated Molecular Weight: 620 aa, 66 kDa Observed Molecular Weight: 66 kDa GenBank Accession Number: BC101799 Gene Symbol: GLIS1 Gene ID (NCBI): 148979 RRID: AB_2879216 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: Q8NBF1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.