Proteintech, 23411-1-AP, DNAJC21 Polyclonal antibody

Size: 20ul / 150ul The DNAJC21 (23411-1-AP) by Proteintech is a Polyclonal antibody targeting DNAJC21 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 23411-1-AP targets DNAJC21 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse brain tissue, HeLa cells, rat brain tissue Positive IP detected in: HEK-293 cells Positive IHC detected in: human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information DNAJC21 is a ubiquitously expressed DnaJ (heat shock protein 40) chaperone protein. Recently DNAJC21 was reported to be implicated in rRNA biogenesis. Loss of DNAJC21 induced abnormal cell morphology and inhibited cell growth. Mutations in DNAJC21 cause bone marrow failure syndrome. (27346687) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20003 Product name: Recombinant human DNAJC21 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 73-307 aa of BC107577 Sequence: EKNELVRQLVAFIRKRDKRVQAHRKLVEEQNAEKARKAEEMRRQQKLKQAKLVEQYREQSWMTMANLEKELQEMEARYEKEFGDGSDENEMEEHELKDEEDGKDSDEAEDAELYDDLYCPACDKSFKTEKAMKNHEKSKKHREMVALLKQQLEEEEENFSRPQIDENPLDDNSEEEMEDAPKQKVKYLTFRFIFALRLSKKQKKKKQKPAQNYDDNFNVNGPGEGVKVDPEDTNL Predict reactive species Full Name: DnaJ (Hsp40) homolog, subfamily C, member 21 Calculated Molecular Weight: 531 aa, 58 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC107577 Gene Symbol: DNAJC21 Gene ID (NCBI): 134218 RRID: AB_2879274 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: Q5F1R6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.