Proteintech, 23712-1-AP, KCNK18 Polyclonal antibody

Size: 20ul / 150ul The KCNK18 (23712-1-AP) by Proteintech is a Polyclonal antibody targeting KCNK18 in WB, IF/ICC, ELISA applications with reactivity to human samples 23712-1-AP targets KCNK18 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, A375 cells, MCF-7 cells Positive IF/ICC detected in: A375 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information KCNK18, also named as TRESK and TRIK, is an outward rectifying potassium channel which produces rapidly activating outward rectifier K+ currents. It may function as background potassium channel that sets the resting membrane potential. KCNK18 is inhibited by arachidonic acid and other naturally occurring unsaturated free fatty acids. KCNK18 channel activity is enhanced by volatile anesthetics, such as isoflurane. The gene of KCNK18 maps to chromosome 10q26.11, and encodes a 384-amino acid protein with a predicted unmodified molecular weight of 44 kDa. This antibody can be used to detect endogenous KCNK18, however, the reason for the additional band of 130-140 kDa is still unknown. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20514 Product name: Recombinant human KCNK18 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 143-290 aa of BC152979 Sequence: LMFLVLTDTGDILATILSTSYNRFRKFPFFTRPLLSKWCPKSLFKKKPDPKPADEAVPQIIISAEELPGPKLGTCPSRPSCSMELFERSHALEKQNTLQLPPQAMERSNSCPELVLGRLSYSIISNLDEVGQQVERLDIPLPIIALIV Predict reactive species Full Name: potassium channel, subfamily K, member 18 Calculated Molecular Weight: 384 aa, 44 kDa Observed Molecular Weight: 44 kDa GenBank Accession Number: BC152979 Gene Symbol: KCNK18 Gene ID (NCBI): 338567 RRID: AB_2879309 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q7Z418 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.