Proteintech, 23718-1-AP, FOXQ1 Polyclonal antibody

Size: 20ul / 150ul The FOXQ1 (23718-1-AP) by Proteintech is a Polyclonal antibody targeting FOXQ1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 23718-1-AP targets FOXQ1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse kidney tissue, rat kidney tissue Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Background Information Forkhead box Q1 (FOXQ1, also known as HFH1), a member of the FOX gene family, contains the core DNA binding domain, whereas the flanking wings of FOXQ1 contribute to its sequence specificity. FOXQ1 is known to repress the promoter activity of smooth muscle-specific genes, such as telokin and SM22α. FOXQ1 expression is regulated by Hoxa1 in embryonic stem cells. [PMID:15722554, 8139574] As a transcription factor, FOXQ1 represses the promoter activity of smooth muscle-specific genes, such as telokin and SM22α, in A10 vascular muscle cells [PMID:10896677]. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20545 Product name: Recombinant human FOXQ1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 188-403 aa of BC053850 Sequence: WGKDNYWMLNPNSEYTFADGVFRRRRKRLSHRAPVPAPGLRPEEAPGLPAAPPPAPAAPASPRMRSPARQEERASPAGKFSSSFAIDSILRKPFRSRRLRDTAPGTTLQWGAAPCPPLPAFPALLPAAPCRALLPLCAYGAGEPARLGAREAEVPPTAPPLLLAPLPAAAPAKPLRGPAAGGAHLYCPLRLPAALQAASVRRPGPHLPYPVETLLA Predict reactive species Full Name: forkhead box Q1 Calculated Molecular Weight: 403 aa, 41 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: BC053850 Gene Symbol: FOXQ1 Gene ID (NCBI): 94234 RRID: AB_2879310 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: Q9C009 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.