Proteintech, 23783-1-AP, PRELP Polyclonal antibody

Size: 20ul / 150ul The PRELP (23783-1-AP) by Proteintech is a Polyclonal antibody targeting PRELP in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 23783-1-AP targets PRELP in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: L02 cells, SMMC-7721 cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information PRELP (proline/arginine-rich end leucine-rich repeat protein), also known as MST161, MSTP161, prolargin, or SLRR2A, belongs to the class II subfamily of the small leucine-rich proteoglycan (SLRP) family. PRELP is a small leucine-rich proteoglycan (SLRP), which binds collagen to basement membranes or cartilage as a matrix protein. PRELP has prognostic value and regulates cell proliferation and migration in hepatocellular carcinoma, may serve as a potential prognostic marker and a treatment target against HCC progression (PMID: 33033521). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20656 Product name: Recombinant human PRELP protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 20-361 aa of BC032498 Sequence: GQPTRRPRPGTGPGRRPRPRPRPTPSFPQPDEPAEPTDLPPPLPPGPPSIFPDCPRECYCPPDFPSALYCDSRNLRKVPVIPPRIHYLYLQNNFITELPVESFQNATGLRWINLDNNRIRKIDQRVLEKLPGLVFLYMEKNQLEEVPSALPRNLEQLRLSQNHISRIPPGVFSKLENLLLLDLQHNRLSDGVFKPDTFHGLKNLMQLNLAHNILRKMPPRVPTAIHQLYLDSNKIETIPNGYFKSFPNLAFIRLNYNKLTDRGLPKNSFNISNLLVLHLSHNRISSVPAINNRLEHLYLNNNSIEKINGTQICPNDLVAFHDFSSDLENVPHLRYLRLDGNY Predict reactive species Full Name: proline/arginine-rich end leucine-rich repeat protein Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 44 kDa GenBank Accession Number: BC032498 Gene Symbol: PRELP Gene ID (NCBI): 5549 RRID: AB_3085714 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P51888 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.