Proteintech, 23938-1-AP, ANK1 Polyclonal antibody

Size: 20ul / 150ul The ANK1 (23938-1-AP) by Proteintech is a Polyclonal antibody targeting ANK1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 23938-1-AP targets ANK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, mouse liver tissue Positive IHC detected in: human stomach cancer tissue, human lymphoma tissue, mouse cerebellum tissue, rat cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information Ankyrin-1 (ANK1) is also named as Ankyrin-R and Erythrocyte ankyrin. The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex (PMID:18440852). ANK1 can attache integral membrane proteins to cytoskeletal elements. And it binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin.(PMID:12456646). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21041 Product name: Recombinant human ANK1 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-155 aa of BC030957 Sequence: MWTFVTQLLVTLVLLSFFLVSCQNVMHIVRGSLCFVLKHIHQELDKELGESEDLSDDEETISTRVVRRRVFLKGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ Predict reactive species Full Name: ankyrin 1, erythrocytic Calculated Molecular Weight: 155 aa, 18 kDa Observed Molecular Weight: 190-250 kDa GenBank Accession Number: BC030957 Gene Symbol: ANK1 Gene ID (NCBI): 286 RRID: AB_3085717 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: P16157 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.