Proteintech, 24058-1-AP, ANKS3 Polyclonal antibody

Size: 20ul / 150ul The ANKS3 (24058-1-AP) by Proteintech is a Polyclonal antibody targeting ANKS3 in WB, IHC, ELISA applications with reactivity to human, mouse samples 24058-1-AP targets ANKS3 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse liver tissue, human brain tissue Positive IHC detected in: human hepatocirrhosis tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information ANKS3 (ankyrin repeat and sterile alpha motif domain containing 3) is a 656 amino acid protein that contain six ANK repeats and one SAM (sterile alpha motif) domain. Each ANK repeat consists of a 30-34 amino acid sequence that exhibits a helix-turn-helix conformation and functions to mediate protein-protein interactions. The SAM domain is a 70 amino acid domain that also functions to mediate protein-protein interactions for homo- and hetero- oligomerization. Two isoforms of ANKS3 exist due to alternative splicing events.This antibody may recognize both of them. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21252 Product name: Recombinant human ANKS3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 179-527 aa of BC050387 Sequence: LEEEGLCCRDVTSPINERDVESSSSSSSREEHAFCANLGPVQSSSSSEGLARAQGLSSEASVESNEDSDHACKSSARKQAKSYMKTKNPDSQWPPRTATDREGFLAESSPQTQRAPYSGPQDLAALLEQIGCLKYLQVFEEQDVDLRIFLTLTESDLKEIGITLFGPKRKMTSAIARWHSSARPPGDALELAYADRLEAEMQELAIQLHKRCEEVEATRGQVCQEQELRAVVESCLLEQDRAREDLQARLRETWALARDAALVLDQLRACQAELSSRVRQDQPPGAATLGLAVPPADSKGWQASLQAMSLPELSGALEDRVREMGQALCLVTQSLEKLQVLSGKKWRET Predict reactive species Full Name: ankyrin repeat and sterile alpha motif domain containing 3 Calculated Molecular Weight: 656 aa, 72 kDa Observed Molecular Weight: 72 kDa, 63 kDa GenBank Accession Number: BC050387 Gene Symbol: ANKS3 Gene ID (NCBI): 124401 RRID: AB_2879418 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: Q6ZW76 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.