Proteintech, 24061-1-AP, SGF29 Polyclonal antibody

Size: 20ul / 150ul The SGF29 (24061-1-AP) by Proteintech is a Polyclonal antibody targeting SGF29 in WB, IHC, ELISA applications with reactivity to human samples 24061-1-AP targets SGF29 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, MDA-MB-453s cells Positive IHC detected in: human liver cancer tissue, human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:800 Background Information SAGA-associated factor 29 (SGF29) is a chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes. SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PMID:19103755, PMID:26421618, PMID:21685874, PMID:26578293). Involved in the response to the endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (PubMed:23894581). It has a calculated molecular weight of 33 kDa. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21261 Product name: Recombinant human CCDC101 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-293 aa of BC011981 Sequence: MALVSADSRIAELLTELHQLIKQTQEERSRSEHNLVNIQKTHERMQTENKISPYYRTKLRGLYTTAKADAEAECNILRKALDKIAEIKSLLEERRIAAKIAGLYNDSEPPRKTMRRGVLMTLLQQSAMTLPLWIGKPGDKPPPLCGAIPASGDYVARPGDKVAARVKAVDGDEQWILAEVVSYSHATNKYEVDDIDEEGKERHTLSRRRVIPLPQWKANPETDPEALFQKEQLVLALYPQTTCFYRALIHAPPQRPQDDYSVLFEDTSYADGYSPPLNVAQRYVVACKEPKKK Predict reactive species Full Name: coiled-coil domain containing 101 Calculated Molecular Weight: 293 aa, 33 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC011981 Gene Symbol: SGF29 Gene ID (NCBI): 112869 RRID: AB_2879420 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: Q96ES7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.