Proteintech, 24127-1-AP, SOD2 Polyclonal antibody

Size: 20ul / 150ul The SOD2 (24127-1-AP) by Proteintech is a Polyclonal antibody targeting SOD2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 24127-1-AP targets SOD2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: DU 145 cells, HeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human liver cancer tissue, human breast cancer tissue, human colon tissue, human hepatocellular carcinomaNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HUVEC cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Superoxide dismutase 2 (SOD2), also known as MnSOD (manganese-dependent superoxide dismutase), is amitochondrial enzyme that dismutates superoxide ions.What is the molecular weight of SOD2? Is SOD2 post-translationally modified?The molecular weight of SOD2 is 25 kDa. SOD2 acts as a homotetramer with manganeseion. SOD2 can bepost-translationally modified, including phosphorylation, nitration, and acetylation. Phosphorylation ofSOD2increases its enzymatic activity and occurs as part of the adaptive response to radiation stress(PMID: 23243068),while nitration and acetylation decrease SOD2 enzymatic activity (PMID: 20106845,21172655).What is the subcellular localization of superoxide dismutases?SOD2 is the only superoxide dismutase that localizes to the mitochondrial matrix (PMID: 4578091). SOD1ispredominantly found in the cytoplasm and to a lesser extent in the mitochondrial intermembrane spaceandnucleus (PMID: 11507097), while SOD3 localizes extracellularly.What is the role of SOD2 in neutralizing reactive oxygen species (ROS)?Superoxide is a main form of ROS that originates directly from theelectron transport chain in the mitochondrialmatrix.SOD2 plays a crucial role in mitochondrial ROS homeostasis by catalyzing turnover of superoxide radicalsto hydrogen peroxide and molecular oxygen. ROS are potent physiological signaling molecules but at high dosesthey contribute to the pathogenesis of many diseases, including cardiovascular and neurodegenerative diseasesand cancer (PMID: 29669742). SOD2 also associates with mitochondrial DNA (mtDNA), protecting it fromoxidativedamage (PMID: 19228881).What is the tissue expression pattern of SOD2?SOD2 is ubiquitously expressed.Can SOD2 be used as a marker of oxidative stress?SOD2 is a key player inantioxidant defenseand contributes to the regulation of oxidative stress. Defects inSOD2activity or levels can result in oxidative stress. However, SOD2 cannot be used as a marker of oxidativestressbecause its expression is not always altered by oxygen stress. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish, bovine, sheep, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21388 Product name: Recombinant human SOD2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-140 aa of BC016934 Sequence: MLSRAVCGTSRQLAPALGYLGSRQKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGRFQAERREAVPGRGDPREPGPIRTGLSVEENSLRICTGSEFSRHDSLSFKHMVYLIVEGVPRWV Predict reactive species Full Name: superoxide dismutase 2, mitochondrial Calculated Molecular Weight: 25 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: BC016934 Gene Symbol: SOD2 Gene ID (NCBI): 6648 RRID: AB_2879437 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P04179 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.