Proteintech, 24337-1-AP, BBOF1 Polyclonal antibody

Size: 20ul / 150ul The BBOF1 (24337-1-AP) by Proteintech is a Polyclonal antibody targeting BBOF1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 24337-1-AP targets BBOF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, TT cells, rat testis tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Basal body orientation factor 1 (BBOF1), also known as CCDC176, contains two coiled-coil domains and is evolutionarily conserved in vertebrates. Recently, Xenopus bbof1 has been characterized as a cilia-associated factor that is induced by the transcription factor foxj1 to mediate the alignment and orientation of cilia in epidermal multi-ciliated cells. BBOF1 is required for sperm flagellar axoneme maintenance and male fertility in mice. BBOF1 expression is elevated during meiosis, and it is accumulated in round spermatids. BBOF1 deletion has compromised effects on growth, survival, and spermatogenesis. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19302 Product name: Recombinant human C14orf45 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 12-229 aa of BC104981 Sequence: LQQHAMIENQAGQVEIDKLQHLLQMKDREMNRVKKLAKNILDERTEVERFFLDALHQVKQQILISRKHYKQIAQAAFNLKMRAACTGRTEYPKIRTFDGREHSTNSVNQDLLEAEKWTHIEGNVDIGDLTWEQKEKVLRLLFAKMNGCPSRKYNQSSRPPVPDYVVSDSGETKEFGDESKLQDKIFITQQIAISDSSGEVVLPTIPKEPQESDTGTF Predict reactive species Full Name: chromosome 14 open reading frame 45 Calculated Molecular Weight: 529 aa, 62 kDa Observed Molecular Weight: 62 kDa GenBank Accession Number: BC104981 Gene Symbol: BBOF1 Gene ID (NCBI): 80127 RRID: AB_3669433 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8ND07 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.