Proteintech, 24362-1-AP, DNER Polyclonal antibody

Size: 20ul / 150ul The DNER (24362-1-AP) by Proteintech is a Polyclonal antibody targeting DNER in WB, IHC, ELISA applications with reactivity to human, mouse, pig samples 24362-1-AP targets DNER in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples. Tested Applications Positive WB detected in: mouse cerebellum tissue, pig cerebellum tissue Positive IHC detected in: mouse cerebellum tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Background Information Delta and Notch-like epidermal growth factor-related receptor (DNER) is a type I transmembrane protein that is specifically expressed in the developing and mature central nervous system (PMID: 11950833). It contains ten extracellular EGF-like repeats that are closely related to those of the developmentally important receptor Notch and its ligand Delta. DNER functions as a Notch ligand and mediates signaling through neuron-glia interactions (PMID: 15965470). Specification Tested Reactivity: human, mouse, pig Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19955 Product name: Recombinant human DNER protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 75-387 aa of BC035009 Sequence: EPGYSCTCPAGISGANCQLVADPCASNPCHHGNCSSSSSSSSDGYLCICNEGYEGPNCEQALPSLPATGWTESMAPRQLQPVPATQEPDKILPRSQATVTLPTWQPKTGQKVVEMKWDQVEVIPDIACGNASSNSSAGGRLVSFEVPQNTSVKIRQDATASLILLWKVTATGFQQCSLIDGRSVTPLQASGGLVLLEEMLALGNNHFIGFVNDSVTKSIVALRLTLVVKVSTCVPGESHANDLECSGKGKCTTKPSEATFSCTCEEQYVGTFCEEYDACQRKPCQNNASCIDANEKQDGSNFTCVCLPGYTGE Predict reactive species Full Name: delta/notch-like EGF repeat containing Calculated Molecular Weight: 78 kDa Observed Molecular Weight: 110-130 kDa GenBank Accession Number: BC035009 Gene Symbol: DNER Gene ID (NCBI): 92737 RRID: AB_3085732 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NFT8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.