Proteintech, 24421-1-AP, TALPID3 Polyclonal antibody

Size: 20ul / 150ul The TALPID3 (24421-1-AP) by Proteintech is a Polyclonal antibody targeting TALPID3 in IHC, IF/ICC, ELISA applications with reactivity to human, mouse, canine samples 24421-1-AP targets TALPID3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples. Tested Applications Positive IHC detected in: human brain tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MDCK cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information KIAA0586 also named as Talpid3 is a 1472 amino-acid protein, which belongs to Talpid3 family. Talpid3 is a gene essential for vertebrate development. Studies in chicken, mouse, zebrafish and human have shown that TALPID3 is a centrosomal protein, mutants null for TALPID3 fail to produce cilia, resulting in far reaching signalling defects such as polydactyly, dorsalised neural tube, craniofacial and vascular defects often due to abnormalities in Hedgehog signal transduction. Talpid3 is absolutely required for the function of both Gli repressor and activator in the intracellular Hedgehog pathway and ciliogenesis. TALPID3 may be identified as a subcellular maker of centrosome and needed to further studied. Specification Tested Reactivity: human, mouse, canine Cited Reactivity: human, mouse, caenorhabditis elegans Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19813 Product name: Recombinant human KIAA0586 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1201-1533 aa of BC066647 Sequence: SQMPGSDSSTLESTLSVTVTETETLDKPISEGEILFSCGQKLAPKILEDIGLYLTNLNDSLSSTLHDAVEMEDDPPSEGQVIRMSHKKFHADAILSFAKQNQESAVSQQAVYHSEDLENSVGELSEGQRPQLTAAAENILMGHSLYMQPPVTNTQSLDQQCDPKPLSRQFDTVSGSIYEDSCASHGPMSLGELELEPNSKLVLPTTLLTAQENDVNLPVAAEDFSQYQLKQNQDVKQVEHKPSQSYLRVRNKSDIAPSQQQVSPGDMDRTQIELNPYLTCVFSGGKAVPLSASQMPPAKMSVMLPSVNLEDCSQSLSLSTMQEDMESSGADTF Predict reactive species Full Name: KIAA0586 Calculated Molecular Weight: 1533 aa, 169 kDa GenBank Accession Number: BC066647 Gene Symbol: TALPID3 Gene ID (NCBI): 9786 RRID: AB_2879539 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BVV6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.