Proteintech, 24479-1-AP, GID8 Polyclonal antibody

Size: 20ul / 150ul GID8 Polyclonal Antibody for WB, IHC, IF-P, ELISA 24479-1-AP targets GID8 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, K-562 cells, HeLa cells, Jurkat cells, mouse brain tissue, rat brain tissue, SW480 cells, HepG2 cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse colon tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:500-1:2000 Background Information The GID8 protein is a component of the Glucose-Induced Degradation-deficient (GID) complex, which plays a key role in ubiquitin-mediated protein degradation, a pathway used to regulate protein turnover in eukaryotic cells. Initially discovered in yeast, GID8 acts as a scaffold protein that is essential for the assembly of the multi-subunit GID complex and for substrate recognition. This complex plays a significant role in metabolic regulation, such as degrading fructose-1,6-bisphosphatase (FBPase) during the shift from gluconeogenesis to glycolysis. In humans, GID8 is part of the CTLH complex, which is associated with various cellular processes including cell cycle regulation, apoptosis, and cancer progression. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19751 Product name: Recombinant human C20orf11 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-228 aa of BC032120 Sequence: MSYAEKPDEITKDEWMEKLNNLHVQRADMNRLIMNYLVTEGFKEAAEKFRMESGIEPSLDLETLDERIKIREMILKGQIQEAIALINSLHPELLDTNRYLYFHLQQQHLIELIRQRETEAALEFAQTQLAEQGEESRECLTEMERTLALLAFDSPEESPFGDLLHTMQRQKVWSEVNQAVLDYENRESTPKLAKLLKLLLWAQNELDQKKVKYPKMTDLSKGVIEEPK Predict reactive species Full Name: chromosome 20 open reading frame 11 Calculated Molecular Weight: 228 aa, 27 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC032120 Gene Symbol: GID8 Gene ID (NCBI): 54994 RRID: AB_2879562 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NWU2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.