Proteintech, 24583-1-AP, PDZ-GEF2 Polyclonal antibody

Size: 20ul / 150ul The PDZ-GEF2 (24583-1-AP) by Proteintech is a Polyclonal antibody targeting PDZ-GEF2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 24583-1-AP targets PDZ-GEF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse brain tissue, HeLa cells Positive IHC detected in: mouse brain tissue, rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information RAPGEF6, also named PDZ-GEF2 enables several functions, including GTP-dependent protein binding activity; guanyl-nucleotide exchange factor activity; and phosphatidic acid binding activity. It is also involved in microvillus assembly; positive regulation of GTPase activity; and protein localization to plasma membrane. Located in several cellular components, including apical plasma membrane; centrosome; and endocytic vesicle. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20325 Product name: Recombinant human PDZ-GEF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-164 aa of BC142964 Sequence: MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYL Predict reactive species Full Name: Rap guanine nucleotide exchange factor (GEF) 6 Calculated Molecular Weight: 1601 aa, 179 kDa Observed Molecular Weight: 179 kDa GenBank Accession Number: BC142964 Gene Symbol: RAPGEF6 Gene ID (NCBI): 51735 RRID: AB_3085740 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TEU7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.