Proteintech, 24758-1-AP, SMC4 Polyclonal antibody

Size: 20ul / 150ul The SMC4 (24758-1-AP) by Proteintech is a Polyclonal antibody targeting SMC4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 24758-1-AP targets SMC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, C6 cells Positive IHC detected in: human colon tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The calculated molecular weight of SMC4 is 147 kDa, but modified SMC4 is about 160 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19156 Product name: Recombinant human SMC4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 992-1288 aa of BC106033 Sequence: SVLIHNSDEHKDIQSCTVEVHFQKIIDKEGDDYEVIPNSNFYVSRTACRDNTSVYHISGKKKTFKDVGNLLRSHGIDLDHNRFLILQGEVEQIAMMKPKGQTEHDEGMLEYLEDIIGCGRLNEPIKVLCRRVEILNEHRGEKLNRVKMVEKEKDALEGEKNIAIEFLTLENEIFRKKNHVCQYYIYELQKRIAEMETQKEKIHEDTKEINEKSNILSNEMKAKNKDVKDTEKKLNKITKFIEENKEKFTQLDLEDVQVREKLKHATSKAKKLEKQLQKDKEKVEEFKSIPAKSNNII Predict reactive species Full Name: structural maintenance of chromosomes 4 Calculated Molecular Weight: 1288 aa, 147 kDa Observed Molecular Weight: 147-150 kDa GenBank Accession Number: BC106033 Gene Symbol: SMC4 Gene ID (NCBI): 10051 RRID: AB_2879707 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NTJ3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.