Proteintech, 24819-1-AP, INO80 Polyclonal antibody

Size: 20ul / 150ul The INO80 (24819-1-AP) by Proteintech is a Polyclonal antibody targeting INO80 in WB, IP, IHC, ELISA applications with reactivity to human samples 24819-1-AP targets INO80 in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells Positive IP detected in: HeLa cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20309 Product name: Recombinant human INO80 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1217-1556 aa of BC146785 Sequence: KQVTVYRLICKGTIEERILQRAKEKSEIQRMVISGGNFKPDTLKPKEVVSLLLDDEELEKKLRLRQEEKRQQEETNRVKERKRKREKYAEKKKKEDELDGKRRKEGVNLVIPFVPSADNSNLSADGDDSFISVDSAMPSPFSEISISSELHTGSIPLDESSSDMLVIVDDPASSAPQSRATNSPASITGSVSDTVNGISIQEMPAAGRGHSARSRGRPKGSGSTAKGAGKGRSRKSTAGSAAAMAGAKAGAAAASAAAYAAYGYNVSKGISASSPLQTSLVRPAGLADFGPSSASSPLSSPLSKGNNVPGNPKNLHMTSSLAPDSLVRKQGKGTNPSGGR Predict reactive species Full Name: INO80 homolog (S. cerevisiae) Calculated Molecular Weight: 1556 aa, 177 kDa Observed Molecular Weight: 177 kDa, 150 kDa GenBank Accession Number: BC146785 Gene Symbol: INO80 Gene ID (NCBI): 54617 RRID: AB_2879741 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9ULG1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.