Proteintech, 24868-1-AP, XBP1S-specific Polyclonal antibody

Size: 20ul / 150ul The XBP1S-specific (24868-1-AP) by Proteintech is a Polyclonal antibody targeting XBP1S-specific in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 24868-1-AP targets XBP1S-specific in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293T cells, MCF-7 cells, mouse liver tissue, rat liver tissue Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information XBP1, also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). XBP1 localizes in the nucleus. As a transcription factor, it is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The calculated molecular weight of protein generated from the unspliced XBP1 isoform XBP1U mRNA is 33 kDa, and protein generated from spliced XBP1 isoform XBP1S mRNA is 40 kDa, and shows at 45-65 kDa range. This antibody specially recognizes the XBP1S isoform. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21703 Product name: Recombinant human XBP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 167-376 aa of BC000938 Sequence: GAGPVVTPPEHLPMDSGGIDSSDSESDILLGILDNLDPVMFFKCPSPEPASLEELPEVYPEGPSSLPASLSLSVGTSSAKLEAINELIRFDHIYTKPLVLEIPSETESQANVVVKIEEAPLSPSENDHPEFIVSVKEEPVEDDLVPELGISNLLSSSHCPKPSSCLLDAYSDCGYGGSLSPFSDMSSLLGVNHSWEDTFANELFPQLISV Predict reactive species Full Name: X-box binding protein 1 Calculated Molecular Weight: 261 aa, 29 kDa Observed Molecular Weight: 45-65 kDa GenBank Accession Number: BC000938 Gene Symbol: XBP1 Gene ID (NCBI): 7494 RRID: AB_2879766 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P17861 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.