Proteintech, 24921-1-AP, KIAA0774 Polyclonal antibody

Size: 20ul / 150ul The KIAA0774 (24921-1-AP) by Proteintech is a Polyclonal antibody targeting KIAA0774 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 24921-1-AP targets KIAA0774 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse heart tissue Positive IHC detected in: human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information KIAA0774, also named as MTUS2, CAZIP and TIP150, binds microtubules. It may regulate the dynamics of microtubules at their growing distal tip. KIAA0774 has three isoforms with MW 150 kDa, 41 kDa and 30 kDa. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20593 Product name: Recombinant human KIAA0774 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1022-1287 aa of BC032481 Sequence: MGHCCCKPYNCLQCLDKTNESALVKEKELSIELANIRDEVAFHTAKCEKLQKEKEELERRFEDEVKRLGWQQQAELQELEERLQLQFEAEMARLQEEHGDQLLSIRCQHQEQVEDLTASHDAALLEMENNHTVAITILQDDHDHKVQELMSTHELEKKELEENFEKLRLSLQDQVDTLTFQSQSLRDRARRFEEALRKNTEEQLEIALAPYQHLEEDMKSLKQVLEMKNQQIHEQEKKILELEKLAEKNIILEEKIQVLQQQNEDL Predict reactive species Full Name: KIAA0774 Calculated Molecular Weight: 1369 aa, 150 kDa Observed Molecular Weight: 150 kDa, 41 kDa GenBank Accession Number: BC032481 Gene Symbol: KIAA0774 Gene ID (NCBI): 23281 RRID: AB_2879797 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5JR59 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.