Proteintech, 25128-1-AP, Cleaved Caspase 3 Polyclonal antibody

Size: 20ul / 150ul The Cleaved Caspase 3 (25128-1-AP) by Proteintech is a Polyclonal antibody targeting Cleaved Caspase 3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 25128-1-AP targets Cleaved Caspase 3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize the cleaved Caspase 3 fragments. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved fragment of Caspase 3 might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, chicken, bovine, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: caspase 3, apoptosis-related cysteine peptidase Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 17-25 kDa GenBank Accession Number: NM_032991 Gene Symbol: Caspase 3 Gene ID (NCBI): 836 RRID: AB_3073913 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P42574 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.