Proteintech, 25149-1-AP, NAT12 Polyclonal antibody

Size: 20ul / 150ul The NAT12 (25149-1-AP) by Proteintech is a Polyclonal antibody targeting NAT12 in WB, IF/ICC, ELISA applications with reactivity to human samples 25149-1-AP targets NAT12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information N-terminal acetylation (Nt-acetylation) by N-terminal acetyltransferases (NATs) is one of the most common protein modifications in eukaryotes. The NatC complex represents one of three major NATs of which the substrate profile remains largely unexplored. NAT12/NAA30 (N-alpha-acetyltransferase 30) is the catalytic subunit of the NAT-C complex (PMID: 27694331, 26292663). The expected sizes of the two isoforms of the protein were 35 and 39 kDa. Western blots detected two bands of NAA12, the expected ~40 kDa band and a possibly dimer at ~70 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18435 Product name: Recombinant human NAT12 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-362 aa of BC118589 Sequence: MAEVPPGPSSLLPPPAPPAPAAVEPRCPFPAGAALACCSEDEEDDEEHEGGGSRSPAGGESATVAAKGHPCLRCPQPPQEQQQLNGLISPELRHLRAAASLKSKVLSVAEVAATTATPDGGPRATATKGAGVHSGERPPHSLSSNARTAVPSPVEAAAASDPAAARNGLAEGTEQEEEEEDEQVRLLSSSLTADCSLRSPSGREVEPGEDRTIRYVRYESELQMPDIMRLITKDLSEPYSIYTYRYFIHNWPQLCFLAMVGEECVGAIVCKLDMHKKMFRRGYIAMLAVDSKYRRNGIGTNLVKKAIYAMVEGDCDEVVLETEITNKSALKLYENLGFVRDKRLFRYYLNGVDALRLKLWLR Predict reactive species Full Name: N-acetyltransferase 12 (GCN5-related, putative) Calculated Molecular Weight: 362 aa, 39 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC118589 Gene Symbol: NAT12 Gene ID (NCBI): 122830 RRID: AB_3085773 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q147X3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.