Proteintech, 25199-1-AP, AKAP12 Polyclonal antibody

Size: 20ul / 150ul The AKAP12 (25199-1-AP) by Proteintech is a Polyclonal antibody targeting AKAP12 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 25199-1-AP targets AKAP12 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: rat brain tissue Positive IHC detected in: mouse brain tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information AKAP12, also named as AKAP250, is a 1782 amino acid protein, which contains three AKAP domains. AKAP12 localizes in the cytoplasm and is expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. AKAP12 as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). AKAP12 expression is known to be suppressed in several human malignancies including breast, prostate, gastric and colon cancers. AKAP12 has three isoforms, namely AKAP12A, AKAP12B and AKAP12C, with molecular weights of 305 kDa, 287 kDa and 250 kDa respectively. (PMID: 30385176, PMID: 15258566) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17769 Product name: Recombinant human AKAP12 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1335-1684 aa of BC157067 Sequence: ETANILETGETLEPAGAHLVLEEKSSEKNEDFAAHPGEDAVPTGPDCQAKSTPVIVSATTKKGLSSDLEGEKTTSLKWKSDEVDEQVACQEVKVSVAIEDLEPENGILELETKSSKLVQNIIQTAVDQFVRTEETATEMLTSELQTQAHVIKADSQDAGQETEKEGEEPQASAQDETPITSAKEESESTAVGQAHSDISKDMSEASEKTMTVEVEGSTVNDQQLEEVVLPSEEEGGGAGTKSVPEDDGHALLAERIEKSLVEPKEDEKGDDVDDPENQNSALADTDASGGLTKESPDTNGPKQKEKEDAQEVELQEGKVHSESDKAITPQAQEELQKQERESAKSELTES Predict reactive species Full Name: A kinase (PRKA) anchor protein 12 Calculated Molecular Weight: 1782 aa, 191 kDa Observed Molecular Weight: 200-300 kDa GenBank Accession Number: BC157067 Gene Symbol: AKAP12 Gene ID (NCBI): 9590 RRID: AB_2879954 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q02952 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.