Proteintech, 25230-1-AP, IFT80 Polyclonal antibody

Size: 20ul / 150ul The IFT80 (25230-1-AP) by Proteintech is a Polyclonal antibody targeting IFT80 in WB, ELISA applications with reactivity to human, mouse samples 25230-1-AP targets IFT80 in WB, IF, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse kidney tissue, NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Background Information IFT80 is an intraflagellar transport (IFT) protein that plays a crucial role in the formation and function of primary cilia. IFT proteins are key for cilia formation, and it seems that this organelle has developed into a complex signaling center in mammals, providing important sensory functions. IFT80 is a recently-defined component of IFT complex B, and loss of IFT80 in zebrafish results in cystic kidneys and a reduced number of cilia, whereas silencing IFT80 in Tetrahymena thermophila produces shortened or absent cilia. (PMID: 23333501) Specification Tested Reactivity: human, mouse Cited Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18650 Product name: Recombinant human IFT80 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 417-777 aa of BC101494 Sequence: LNAQTVSLSNDTIAIRDKADEKIIFLFEASTGKPLGDGKFLSHKNEILEIALDQKGLTNDRKIAFIDKNRDLCITSVKRFGKEEQIIKLGTMVHTLAWNDTCNILCGLQDTRFIVWYYPNTVYVDRDILPKTLYERDASEFSKNPHIVSFVGNQVTIRRADGSLVHISITPYPAILHEYVSSSKWEDAVRLCRFVKEQTMWACLAAMAVANRDMTTAEIAYAAIGEIDKVQYINSIKNLPSKESKMAHILLFSGNIQEAEIVLLQAGLVYQAIQININLYNWERALELAVKYKTHVDTVLAYRQKFLETFGKQETNKRYLHYAEGLQIDWEKIKAKIEMEITKEREQSSSSQSSKSIGLKP Predict reactive species Full Name: intraflagellar transport 80 homolog (Chlamydomonas) Calculated Molecular Weight: 777 aa, 88 kDa Observed Molecular Weight: 88-90 kDa GenBank Accession Number: BC101494 Gene Symbol: IFT80 Gene ID (NCBI): 57560 RRID: AB_2879976 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P2H3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.