Proteintech, 25239-1-AP, TDRD5 Polyclonal antibody

Size: 20ul / 150ul The TDRD5 (25239-1-AP) by Proteintech is a Polyclonal antibody targeting TDRD5 in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples 25239-1-AP targets TDRD5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse testis tissue Positive IP detected in: mouse testis tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TDRD5, also named as Tudor domain-containing protein 5, is a 981 amino acid protein, which contains one Tudor domain and three HTH OST-type domains. TDRD5 localizes in the cytiplasm and belongs to the TDRD5 family. TDRD5 is required during spermiogenesis to participate in the repression transposable elements and prevent their mobilization, which is essential for the germline integrity. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18896 Product name: Recombinant human TDRD5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-350 aa of BC130532 Sequence: MSEQERIQECLRKEIRSLLISTKDGLSPQELEKEYLLMVGNHLPLRILGYRSTMELVLDMPDVVRVCPGAGGTVILKAIPDESTKGIASLVAKQRSSHKLRNSMHKGRPSIYSGPRSHRRVPYRGSVAPILPAVVKSELKDLLALSPVLLSDFEKAFAKRFGRSFQYMQYGFLSMFEVLNAASDVISVEQTRAGSLLMLKKSVTEEKPRGCPAGKIFTQPFRMKQGSYSTGFPVAKPCFSQPTSNMEPPKQIMSMEKTSKLNVVETSRLNHTEKLNQLENTFKSVIAQIGPGGTISSELKHKIKFVVSKFPEGLFISKLLGEYEVIFKEQLSPKKLGFLNVTELVGALSD Predict reactive species Full Name: tudor domain containing 5 Calculated Molecular Weight: 981 aa, 110 kDa Observed Molecular Weight: ~116 kDa GenBank Accession Number: BC130532 Gene Symbol: TDRD5 Gene ID (NCBI): 163589 RRID: AB_2879981 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NAT2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.