Proteintech, 25342-1-AP, CPEB4 Polyclonal antibody

Size: 20ul / 150ul The CPEB4 (25342-1-AP) by Proteintech is a Polyclonal antibody targeting CPEB4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 25342-1-AP targets CPEB4 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SH-SY5Y cells, A375 cells, HeLa cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CPEB4 also named as CPE binding protein 4 or hCPEB 4 is a 729 amino acid protein, which contains two RRM domains and belongs to the RRM CPEB family. CPEB4 involves in some forms of synaptic plasticity and regulate translation by binding to regulatory motifs of defined mRNA targets. CPEB1 and CPEB4 are differentially regulated by phase-specific kinases, generating the need of two sequential CPEB activities to sustain cytoplasmic polyadenylation during all the meiotic phases. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cytoplasmic polyadenylation element binding protein 4 Calculated Molecular Weight: 80 kDa Observed Molecular Weight: 80 kDa GenBank Accession Number: NM_030627 Gene Symbol: CPEB4 Gene ID (NCBI): 80315 RRID: AB_2880036 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q17RY0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.