Proteintech, 25360-1-AP, ZFYVE28 Polyclonal antibody

Size: 20ul / 150ul The ZFYVE28 (25360-1-AP) by Proteintech is a Polyclonal antibody targeting ZFYVE28 in WB, IF/ICC, ELISA applications with reactivity to human samples 25360-1-AP targets ZFYVE28 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells Positive IF/ICC detected in: HepG2 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information ZFYVE28, also known as LST2, belongs to the lst-2 family. ZFYVE28 is a FYVE domain protein that has been shown to be a regulator of the epidermal growth factor receptor (EGFR) pathway. The non-ubiquitinylated form of ZFYVE28 co-localizes with EGFR in endosomes and promotes receptor degradation (PMID: 29549365). ZFYVE28 has 8 isoforms with the molecular mass of 19-33, 83-93, and 96 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19796 Product name: Recombinant human ZFYVE28 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 575-887 aa of BC137309 Sequence: EDVVERLREKCSPGGVIGASYAAGLAKASDRAPERQEEAPPPSEDASNGREPKAPTSDKCLPHTSGSQVDTASGLQGEAGVAGQQEPEARELHAGSPPAHEAPQALSGSSSSTAGSCSSDKMGPEAAPAATHAAPQATREKIRSRFHGSHDLIHRLFVCISGVADQLQTNYASDLRSILKTLFEVMATKPETDDKEKLRKVTQTLRSAALEDCALCQETLSSSELAAKTRDGDFEDPPEWVPDEACGFCTACKAPFTVIRRKHHCRSCGKIFCSRCSSHSAPLPRYGQVKPVRVCTHCYMFHVTPFYSDKAGL Predict reactive species Full Name: zinc finger, FYVE domain containing 28 Calculated Molecular Weight: 887 aa, 96 kDa Observed Molecular Weight: 96 kDa GenBank Accession Number: BC137309 Gene Symbol: ZFYVE28 Gene ID (NCBI): 57732 RRID: AB_3085790 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9HCC9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.