Proteintech, 25379-1-AP, GFRA3 Polyclonal antibody

Size: 20ul / 150ul The GFRA3 (25379-1-AP) by Proteintech is a Polyclonal antibody targeting GFRA3 in WB, IHC, ELISA applications with reactivity to human, mouse samples 25379-1-AP targets GFRA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse heart tissue Positive IHC detected in: human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information GFRA3 is a receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). It mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. GFRA3 has two isoforms with MW 45 kDa and 41 kDa. It can be cleaved to be a mature form with MW 41 kDa or 37 kDa. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13253 Product name: Recombinant human GFRA3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 38-379 aa of BC037951 Sequence: SRLMNSCLQARRKCQADPTCSAAYHHLDSCTSSISTPLPSEEPSVPADCLEAAQQLRNSSLIGCMCHRRMKNQVACLDIYWTVHRARSLGNYELDVSPYEDTVTSKPWKMNLSKLNMLKPDSDLCLKFAMLCTLNDKCDRLRKAYGEACSGPHCQRHVCLRQLLTFFEKAAEPHAQGLLLCPCAPNDRGCGERRRNTIAPNCALPPVAPNCLELRRLCFSDPLCRSRLVDFQTHCHPMDILGTCATEQSRCLRAYLGLIGTAMTPNFVSNVNTSVALSCTCRGSGNLQEECEMLEGFFSHNPCLTEAIAAKMRFHSQLFSQDWPHPTFAVMAHQNENPAVRP Predict reactive species Full Name: GDNF family receptor alpha 3 Calculated Molecular Weight: 45 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC037951 Gene Symbol: GFRA3 Gene ID (NCBI): 2676 RRID: AB_2880050 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60609 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.