Proteintech, 25566-1-AP, ALKBH8 Polyclonal antibody

Size: 20ul / 150ul The ALKBH8 (25566-1-AP) by Proteintech is a Polyclonal antibody targeting ALKBH8 in IHC, IF/ICC, ELISA applications with reactivity to human, rat samples 25566-1-AP targets ALKBH8 in IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive IHC detected in: rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells, HEK-293 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ALKBH8 (Alpha-ketoglutarate-dependent dioxygenase) is unique among the ALKBH family, since it harbors a 2-oxoglutarate and Fe(II)-dependent oxygenase domain as well as an RNA recognition motif and methyl transferase domain (PMID: 27329810). ALKBH8 is a "writer" enzyme that modifies the wobble uridine of tRNA and regulates protein translation, including that of selenoproteins (PMID: 38550277). ALKBH8 is highly expressed in high-grade, superficially and deeply invasive bladder cancer. ALKBH8 knockdown induces apoptosis in bladder cancer cells (PMID: 38550277). Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22239 Product name: Recombinant human ALKBH8 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-238 aa of BC015183 Sequence: MDSNHQSNYKLSKTEKKFLRKQIKAKHTLLRHEGIETVSYATQSLVVANGGLGNGVSRNQLLPVLEKCGLVDALLMPPNKPYSFARYRTTEESKRAYVTLNGKEVVDDLGQKITLYLNFVEKVQWKELRPQALPPGLMVVEEIISSEEEKMLLESVDWTEDTDNQNSQKSLKHRRVKHFGYEFHYENNNVDKDKPLSGGLPDICESFLEKWLRKGYIKHKPDQMTINQYEPGQDCHGF Predict reactive species Full Name: alkB, alkylation repair homolog 8 (E. coli) Calculated Molecular Weight: 664 aa, 75 kDa GenBank Accession Number: BC015183 Gene Symbol: ALKBH8 Gene ID (NCBI): 91801 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96BT7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.