Proteintech, 25578-1-AP, RPUSD1 Polyclonal antibody

Size: 20ul / 150ul The RPUSD1 (25578-1-AP) by Proteintech is a Polyclonal antibody targeting RPUSD1 in WB, IF/ICC, IP, ELISA applications with reactivity to human samples 25578-1-AP targets RPUSD1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: U2OS cells Positive IP detected in: HeLa cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information RPUSD1 (RNA pseudouridylate synthase domain-containing protein 1) also known as RLUCL (Ribosomal large subunit pseudouridine synthase C-like protein), was significantly upregulated in HCC and expressed at higher levels in advanced stage or poorly differentiated HCC tissues (PMID: 36437949). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22314 Product name: Recombinant human RPUSD1 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-312 aa of BC011783 Sequence: MEPGSVENLSIVYRSRDFLVVNKHWDVRIDSKAWRETLTLQKQLRYRFPELADPDTCYGFRFCHQLDFSTSGALCVALNKAAAGSAYRCFKERRVTKAYLALLRGHIQESRVTISHAIGRNSTEGRAHTMCIEGSQGCENPKPSLTDLVVLEHGLYAGDPVSKVLLKPLTGRTHQLRVHCSALGHPVVGDLTYGEVSGREDRPFRMMLHAFYLRIPTDTECVEVCTPDPFLPSLDACWSPHTLLQSLDQLVQALRATPDPDPEDRGPRPGSPSALLPGPGRPPPPPTKPPETEAQRGPCLQWLSEWTLEPDS Predict reactive species Full Name: RNA pseudouridylate synthase domain containing 1 Calculated Molecular Weight: 312 aa, 35 kDa Observed Molecular Weight: 34~35 kDa GenBank Accession Number: BC011783 Gene Symbol: RPUSD1 Gene ID (NCBI): 113000 RRID: AB_3669487 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UJJ7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.