Proteintech, 25697-1-AP, TACC3 Polyclonal antibody

Size: 20ul / 150ul The TACC3 (25697-1-AP) by Proteintech is a Polyclonal antibody targeting TACC3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 25697-1-AP targets TACC3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Caco-2 cells, HeLa cells, SKOV-3 cells Positive IHC detected in: human cervical cancer tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information TACC3, also known as ERIC 1, belongs to the TACC family. TACC family members TACC1, TACC2, and TACC3 map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. It plays a role in the microtubule-dependent coupling of the nucleus and the centrosome and involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22516 Product name: Recombinant human TACC3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 141-330 aa of BC111771 Sequence: SSESGPGALADLDCSSSSQSPGSSENQMVSPGKVSGSPEQAVEENLSSYSLDRRVTPASETLEDPCRTESQHKAETPHGAEEECKAETPHGAEEECRHGGVCAPAAVATSPPGAIPKEACGGAPLQGLPGEALGYPAGVGTPVPADSTQTLTCAHTSAPESTAPTNHLVAGRAMTLSPQEEVAAGQMASS Predict reactive species Full Name: transforming, acidic coiled-coil containing protein 3 Calculated Molecular Weight: 838 aa, 91 kDa Observed Molecular Weight: 160 kDa GenBank Accession Number: BC111771 Gene Symbol: TACC3 Gene ID (NCBI): 10460 RRID: AB_2880199 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y6A5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.