Proteintech, 25713-1-AP, TTC7B Polyclonal antibody

Size: 20ul / 150ul The TTC7B (25713-1-AP) by Proteintech is a Polyclonal antibody targeting TTC7B in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 25713-1-AP targets TTC7B in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue Positive IP detected in: K-562 cells Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TTC7B (Tetratricopeptide Repeat Domain 7B) is a protein-coding gene belonging to the tetratricopeptide repeat (TPR) family, which plays critical roles in cellular signaling, membrane dynamics, and disease pathogenesis. TTC7B is associated with focal adhesion (FA) disassembly and cell migration, promoting tumor invasion and metastasis. Its expression is tied to FA-related pathways like the PINCH-ILK-PARVIN complex (PMID: 37990988). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22388 Product name: Recombinant human TTC7B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 96-318 aa of BC153122 Sequence: LQESNLIMAKLNYVEGDYKEALNIYARVGLDDLPLTAVPPYRLRVIAEAYATKGLCLEKLPISSSTSNLHVDREQDVITCYEKAGDIALLYLQEIERVILSNIQNRSPKPGPAPHDQELGFFLETGLQRAHVLYFKNGNLTRGVGRFRELLRAVETRTTQNLRMTIARQLAEILLRGMCEQSYWNPLEDPPCQSPLDDPLRKGANTKTYTLTRRARVYSGENI Predict reactive species Full Name: tetratricopeptide repeat domain 7B Calculated Molecular Weight: 843 aa, 94 kDa Observed Molecular Weight: 50-53 kDa GenBank Accession Number: BC153122 Gene Symbol: TTC7B Gene ID (NCBI): 145567 RRID: AB_2880205 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86TV6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.