Proteintech, 25718-1-AP, FAM135A Polyclonal antibody

Size: 20ul / 150ul The FAM135A (25718-1-AP) by Proteintech is a Polyclonal antibody targeting FAM135A in IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 25718-1-AP targets FAM135A in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IHC detected in: mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells, U2OS cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information FAM135A (family with sequence similarity 135) also known as KIAA1411, is a gene in the RP25 locus that is situated between D6S1557 and D6S421. FAM135A is expressed in the retina, brain, skeletal muscle, pancreas, and lung, according to gene expression studies. FAM135A is linked to several tumor-related pathways and is strongly correlated with breast cancer cell invasion and migration (PMID: 36943627). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22472 Product name: Recombinant human FAM135A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC131782 Sequence: MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPSRIPHRVEASLLHATGMTLAFPASVHDSLICSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEMNFLLSLDLHFTDGDYSADDLNALQLISSRTLKLHFSPHRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSVIPTLESVVFGINYTKQLSPDGCSFIIADSFLHHAYRFHYTLCATLLLAFKGLHSYFITVTEEIPSCQKLELEEMDVEARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRILLAQEHHTLRVRRFSEA Predict reactive species Full Name: family with sequence similarity 135, member A Calculated Molecular Weight: 1515 aa, 170 kDa GenBank Accession Number: BC131782 Gene Symbol: FAM135A Gene ID (NCBI): 57579 RRID: AB_3669496 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P2D6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.