Proteintech, 25741-1-AP, ZCCHC2 Polyclonal antibody

Size: 20ul / 150ul The ZCCHC2 (25741-1-AP) by Proteintech is a Polyclonal antibody targeting ZCCHC2 in IP, ELISA applications with reactivity to human samples 25741-1-AP targets ZCCHC2 in IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive IP detected in: HeLa cells Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information ZCCHC2 is a poorly characterized protein of 126 kDa with long intrinsically disordered regions, a PX domain, and a CCHC-type zinc-finger (ZnF) domain. ZCCHC2 is distantly related to ZCCHC14 but lacks the SAM domain that is known to interact with the CNGGN pentaloop in 1E and PRE. ZCCHC2 is a critical host factor for K5. ZCCHC2 recruits the terminal nucleotidyl transferase TENT4 to elongate poly(A) tails with mixed sequences, delaying deadenylation. The observed molecular weight is consistent with what has been described in the literature (PMID: 37413987). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22744 Product name: Recombinant human ZCCHC2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 13-239 aa of BC006340 Sequence: REQERVYEWFGLVLGSAQRLEFMCGLLDLCNPLELRFLGSCLEDLARKDYHYLRDSEAKANGLSDPGPLADFREPAVRSRLIVYLALLGSENREAAGRLHRLLPQVDSVLKSLRAARGEGSRGGAEDERGEDGDGEQDAEKDGSGPEGGIVEPRVGGGLGSRAQEELLLLFTMASLHPAFSFHQRVTLREHLERLRAALRGGPEDAEVEVEPCKFAGPRAQVRRTEP Predict reactive species Full Name: zinc finger, CCHC domain containing 2 Calculated Molecular Weight: 1178 aa, 126 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: BC006340 Gene Symbol: ZCCHC2 Gene ID (NCBI): 54877 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9C0B9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.