Proteintech, 25818-1-AP, c-Cbl Polyclonal antibody

Size: 20ul / 150ul The c-Cbl (25818-1-AP) by Proteintech is a Polyclonal antibody targeting c-Cbl in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 25818-1-AP targets c-Cbl in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Daudi cells, K-562 cells, Raji cells, Ramos cells, Romas cells Positive IP detected in: K-562 cells Positive IHC detected in: human tonsillitis tissue, human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag23033 Product name: Recombinant human CBL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 578-906 aa of BC132733 Sequence: LPPVPSSRLGDSWLPRPIPKVPVSAPSSSDPWTGRELTNRHSLPFSLPSQMEPRPDVPRLGSTFSLDTSMSMNSSPLVGPECDHPKIKPSSSANAIYSLAARPLPVPKLPPGEQCEGEEDTEYMTPSSRPLRPLDTSQSSRACDCDQQIDSCTYEAMYNIQSQAPSITESSTFGEGNLAAAHANTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQVPERPPKPFPRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT Predict reactive species Full Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence Calculated Molecular Weight: 906 aa, 100 kDa Observed Molecular Weight: 100-120 kDa GenBank Accession Number: BC132733 Gene Symbol: CBL Gene ID (NCBI): 867 RRID: AB_2880252 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P22681 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.