Proteintech, 25940-1-AP, HAL Polyclonal antibody

Size: 20ul / 150ul The HAL (25940-1-AP) by Proteintech is a Polyclonal antibody targeting HAL in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 25940-1-AP targets HAL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, rat liver tissue Positive IHC detected in: human skin tissue, mouse skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information HAL, also named as Histidase and HIS, catalyzes the nonoxidative elimination of the α-amino group of histidine. It is closely related to the important plant enzyme phenylalanine ammonia-lyase. HAL has some isoforms with MW 73 kDa, 65 kDa and 49 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22985 Product name: Recombinant human HAL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC096097 Sequence: MPRYTVHVRGEWLAVPCQDAQLTVGWLGREAVRRYIKNKPDNGGFTSVDDAHFLVRRCKGLGLLDNEDRLEVALENNEFVEVVIEGDAMSPDFIPSQPEGVYLYSKYREPEKYIELDGDRLTTEDLVNLGKGRYKIKLTPTAEKRVQKSREVIDSIIKEKTVVYGITTGFGKFARTVIPINKLQELQVNLVRSHSSGVGKPLSPERCRMLLALRINVLAKGYSGISLETLKQVIEMFNASCLPYVPEKGTVGASGDLAPLSHLALGLVGEGKMWSPKSGWADAKYVLEAHGLKPVILKPKEGLALINGTQMITSLGCEAVERASAIARQADIVAALTLEVLKGTTKAFDTDIHAL Predict reactive species Full Name: histidine ammonia-lyase Calculated Molecular Weight: 657 aa, 73 kDa Observed Molecular Weight: 73 kDa GenBank Accession Number: BC096097 Gene Symbol: HAL Gene ID (NCBI): 3034 RRID: AB_2880303 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P42357 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.